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. Author manuscript; available in PMC: 2015 Feb 1.
Published in final edited form as: Nat Neurosci. 2013 Dec 22;17(2):280–288. doi: 10.1038/nn.3613

Figure 3. Paired recordings identify convergent glomerular inputs.

Figure 3

a, An example paired recording from a GFP+ Mz671 neuron and one of its presynaptic PNs. Top trace is a single PN spike evoked by direct current injection. Bottom traces are postsynaptic membrane potentials in individual trials (thicker line is the trial-averaged response). Vertical scale for postsynaptic responses is the same as panel b.

b, Results from multiple experiments of this type. In these experiments, both the pre- and postsynaptic neuron were GFP+. A single row shows trial-averaged postsynaptic responses from five paired recordings, each in a different brain. Shown at left are z-projections of confocal stacks through the antennal lobe displaying the dendritic tufts of biocytin-filled PNs (scale bars 10 μm), together with the total number of connections observed and total number of pairs recorded. DM1 consistently evoked larger responses than either DM2 or DM4 (1-way ANOVA followed by post hoc unpaired two-tailed t-tests, P<0.05 for both comparisons). Note that some responses have a transient peak (arrow) which likely reflects a contribution of voltage-gated postsynaptic conductances. Data are not shown for the following glomeruli, none of which were connected: VA4 (0/1), VC1 (0/3), VC2 (0/3), VL2A (0/1), VM2 (0/2), VM7 (0/2).