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. Author manuscript; available in PMC: 2014 Apr 21.
Published in final edited form as: Diabetes Metab Res Rev. 2014 Jan;30(1):23–30. doi: 10.1002/dmrr.2451

Figure 2.

Figure 2

(A) Real-time RT-PCR analysis of Pax3 mRNA from E 8.5 embryos of mice treated with phosphate-buffered saline (PBS) or 5-aminoimidazole-4-carboxamide-1-beta-4-ribofuranoside (AICAR) on E 7.5 or metformin from E 0.5 to 8.5. Pax3 mRNA was normalized to rRNA and expressed relative to Pax3 mRNA of embryos from PBS-treated pregnancies. ***p <0.001 versus PBS, metformin. (B) Neural tube defects (NTD) of E 10.5 embryos of mice treated with PBS or AICAR on E 7.5 or metformin from E 0.5 to 10.5. NTD are expressed as the per cent of embryos in each litter with an NTD. ***p <0.001 versus PBS, metformin. n = 9 replicate litters for each treatment