CCL11 has no effect on ASM survival. (A) representative photographs showing DAPI staining of nuclear morphology under control conditions, following incubation with: 100 ng/ml CCL11 for 24 h, both showing normal nuclear morphology, or STS as a positive control, (B) percentage of apoptotic cells in each condition, n = 8 donors, (C) detection of annexin V+/PI− and annexin V+/PI+ ASM cells using two colour flow cytometry, n = 9 donors. Data are presented as mean ± SEM. Statistical differences were assessed using unpaired t-tests.