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. Author manuscript; available in PMC: 2014 Apr 21.
Published in final edited form as: J Immunol. 2008 Aug 15;181(4):2772–2780. doi: 10.4049/jimmunol.181.4.2772

FIGURE 2.

FIGURE 2

HLMC proliferation in coculture with ASM is dependent on SCF and IL-6. A, Human ASM expresses membrane-bound SCF, (i) positive immunofluorescence for SCF (green) in ASM monolayers. Representative histogram of SCF expression on ASM by flow cytometry (ii) and geometric mean analysis of ASM demonstrating surface SCF expression (iii), n = 8 experiments. B, Effect of cytokine neutralization. HLMC were cultured in media alone (black line) or cocultured with ASM in the absence SCF/IL-6/FCS (red line), and in the presence of the neutralizing Abs to IL-6 (blue line), SCF (green line), or both (purple line), n = 5 experiments using three HLMC donors and five ASM donors. **, p < 0.01; ***, p < 0.001 by one-way ANOVA. †, p < 0.05 by Tukey’s multiple comparison test comparing cocultured HLMC to addition of neutralizing Abs singularly and in combination.