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Enhanced ASM contractility in response to β-tryptase. Example photographs (A) and time course of contraction of collagen gels impregnated with ASM with or without β-tryptase (0.32 μg/ml) with or without leupeptin (6.7 μg/ml) for 48 h (n = 5 ASM donors) (B), followed by subsequent histamine (100 μM) stimulation to the same gels compared with appropriate control analyzed using ImageJ (C). *, p < 0.05.
