Three-color fluorescence in situ hybridization analysis on sperm before and after radiotherapy of a seminona patient. Aqua color- chromosome 18, red color-Y chromosome, green color-X chromosome. Normal sperm karyotype: 18, X (A) and 18, Y (B), chromosome aneuploidy: 1818, X (C), 1818, Y (D), 18, XX (E), 18, XY (F), 18, YY (G). Chromosome diploidy: 1818, XX (H), 1818, XY (I), 1818, YY (J).