Skip to main content
. 2014 Feb 27;110(8):2063–2071. doi: 10.1038/bjc.2014.105

Figure 3.

Figure 3

ALDH activity and correlation with CD24/CD44 or colony-forming efficiency. (A) FACS profiles of ALDH activity in MCF7 and MDAMB468 cells grown as monolayers. The insets show fluorescence values in the presence of the inhibitor DEAB, used to set the R3 gate. (B) MDAMB468 cells were FACS-sorted into populations with the highest or lowest ALDH activity and stained for CD24 and CD44. (C) Colony-forming efficiencies of the FACS-sorted cells. (D) The percentages of MDAMB468 cells showing ALDH activity after growth as monolayers or under mammosphere growth conditions.