Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Mar 4;110(8):2011–2020. doi: 10.1038/bjc.2014.112

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2014 Cancer Research UK

From twelve months after its original publication, this work is licensed under the Creative Commons Attribution-NonCommercial-Share Alike 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/

PMC Copyright notice

(A and B) Transfection efficiency of negative control FAM-siRNAs in AGS cells. (A) Observation under a regular microscope. (B) Observation of the same field of view using a fluorescence microscope. (A and B: × 200 magnification). (C and D) The effect of different siRNAs on the expression of Lgr5 mRNA and protein. Confluent gastric cancer AGS cells were seeded into 6-well plates, and transfected with different siRNAs (siRNA-Lgr5-409, siRNA-Lgr5-1555, and siRNA-Lgr5-2664) or scramble siRNA (negative control). The untransfected cells served as a blank control. Leucine-rich repeat-containing G-protein-coupled receptor 5 mRNA and protein levels were determined by qPCR and western blotting. Transfection of these Lgr5 siRNAs resulted in a significant decrease in Lgr5 mRNA and protein expression. The Lgr5-homo-2664 exerted the greatest effects in suppressing Lgr5 expression. Triplicate experiments were performed with almost identical results. Expression levels of Lgr5 mRNA are presented as mean±s.d.