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Aza-dC downregulates the expression of stem cell-associated genes in pluripotent stem cells through DNMT3B. Expression of OCT4, NANOG and SOX2 was assessed by RT-qPCR upon treatments by doxycycline (Dox) to knockdown DNMT3B and by Aza-dC in (A) N2102Ep, (B) TERA1, (C) NTERA2 and (D) H7 ES cells. Data are represented as mean±s.d.; n=3; *P<0.05.
