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. 2014 Mar 6;110(8):2131–2138. doi: 10.1038/bjc.2014.128

Figure 5.

Figure 5

Nullipotent EC cells are more sensitive to Aza-dC than their differentiated cells. (A) Western blotting of N2102Ep and TERA1 clones with OCT4 silencing. Expression of DNMT3B was also downregulated by silencing of OCT4. (B) Expression of differentiation-associated genes was upregulated in OCT4 knockdown EC cells. (C) Dose response of Aza-dC on cell number of EC cells N2102Ep and TERA1 upon OCT4 silencing was determined. Cells were cultured for 7 days with new culture medium containing indicated concentration of Aza-dC replaced every day. Data are represented as mean±s.d.; n=3.