Figure 4.

PR cells have high expression of Skp2. (A) Real-time RT–PCR assay was conducted to detect the expression of Skp2 in parental and PR cells. *P<0.05 PR vs control. (B) Western blotting analysis was performed to detect the expression of Skp2 and its target genes in parental and PR cells. (C) Cell cycle analysis was performed by PI staining and flow cytometry in parental and PR cells. (D) Western blotting analysis was performed to detect the efficacy of Skp2 siRNA transfection. (E) Cell morphology was taken by microscopy in PR cells transfected with Skp2 siRNA. (F) Cell attachment and detachment assays were measured in PR cells transfected with Skp2 siRNA. *P<0.05 vs control. Abbreviations: CS=control siRNA; SS=Skp2 siRNA.