Figure 6.

PPP2CA restoration decreases incidence of metastasis. (A) Liver (upper), lungs (middle) and lumber lymph nodes (lower) from mice were excised out and fixed in Bouin's solution and number of metastatic colonies were counted (in case of liver and lungs) and total mass of all the lumber lymph nodes was taken. Data presented as number of metastatic modules per mouse in case of liver and lungs and, total weight of the lumber lymph nodes per mouse. Dark line indicates the median value of the group. (B) Haematoxylin and eosin staining of liver, lung, lymph node and bone sections from control and PPP2CA-overexpressing group. Mice group injected with PC3-Neo cells show metastatic deposits of cancer cells in liver, lung, lymph node and bone, whereas PPP2CA-overexpressing group had no gross evidence of deposits of cancer cells. Abbreviations: N=normal; T=tumour.