Figure 1.

Strategy for siRNA validation against the Mut Brtl Col1a1 transcript. (a) Alignment of the sequences of the WT and Mut murine Col1a1 (NM_007742.3) exon 23. Nucleotide variations between WT and Mut sequences are shown in larger characters and the one causing the glycine 349 substitution to cysteine is in bold (b). Sense strands of 19 nucleotides are indicated for the six customer-designed Mut siRNAs to target the sequence around the causative mutation and the WT siRNA. (c) Representation of the recombinant transcripts coding the firefly luciferase and containing either WT or Mut Col1a1 exon 23 sequences (black boxes) in its 3' untranslated region, named recombinant WT-LucF or Mut-LucF transcript, respectively. The number of mismatches between the Mut siRNAs and Col1a1 sequences is indicated.