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. 2013 Sep 11;22(5):667–674. doi: 10.1038/ejhg.2013.198

Figure 4.

Figure 4

Evaluation at protein level of collagen chains and HSP47 expression in F-Mut shRNA-expressing Brtl fibroblasts. (a) Representative SDS-PAGE of type I collagen from medium and cell lysates obtained from untransduced and LacZ and F-Mut shRNA-transduced Brtl fibroblasts. (b) Densitometric analysis of the Mut α1(I)–α1(I) dimer percentage as represented in panel a. The Mut collagen was significantly reduced only in Brtl fibroblasts transduced with F-Mut shRNA both in medium and cell layer fractions. The reported values are expressed as mean±SD of three independent experiments. (c) Representative western blot of HSP47 performed on cell lysates obtained from untransduced and LacZ and F-Mut shRNA-transduced Brtl fibroblasts. α tubulin is used as loading control. (d) Densitometry of HSP47 with respect to α tubulin as detected by western blot. HSP47 expression was reduced significantly only in Brtl fibroblasts transduced with F-Mut shRNA. The reported values are expressed as mean±SD of three independent experiments. *P<0.05 referred to F-Mut shRNA-transduced cells versus untransduced fibroblasts and LacZ shRNA-transduced cells.