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Sorting of newly synthesized cathepsin D in control and ML fibroblasts GM01586 (ML II), patient 2 (K4Q compound heterozygote), and GM00113 (K4Q homozygote). Autoradiographs of 35S-labeled immunoprecipitates of cell and secreted cathepsin D are shown. The percentage of intracellular enzyme is determined by dividing the amount of cell-associated cathepsin D by the total cathepsin D (cell-associated and secreted).
