Fig. 5.

(A) Heat shock tolerance and (B) zymolyase resistance in immobilized versus planktonic yeast. Survivorship following exposure to 48 °C was estimated as the ratio of CFUs to total cell number enumerated by hemocytometry. Digestion of the cell wall was followed by a change in optical density at 660 nm. Open triangle, planktonic stationary phase; closed triangle, planktonic log phase; closed circle, immobilized cells in calcium–alginate matrix; open circle, immobilized cells released from the matrix.