Fig. 2.

Specific binding between the receptor ScbR2 and JdB in vitro. (A) Gel mobility shift assays of the DNA-binding activity of ScbR2 in the presence of various antibiotics. The assays were performed in 0.3 μM ScbR2, with 6.6 ng (0.13 nM) of PscbA or 6.6 ng (0.08 nM) of PkasO probes. Amp, ampicillin; Ery, erythromycin; Kan, kanamycin; Tet, tetracycline. (B) ITC analysis of the binding of ScbR2 to JdB. ScbR2 (100 μM) was injected into a solution of 20 μM JdB. (Upper) Heat changes measured as a function of time at 25 °C are shown. (Lower) Normalized heat changes (black square) and the best-fit curve (solid line) and calculated parameters are shown. (Inset) The estimated binding stoichiometry constant and enthalpy are shown.