Fig. 4.

Binding of ScbR2 with PredD and PadpA in vitro and in vivo. (A and B) Gel mobility shift assays of the binding of ScbR2 to PredD (A) and PadpA (B). The three probes (PredD, PadpA, and PhrdB) were used at a concentration of 0.12 nM in the assays. SSDNA, random fragments of salmon sperm DNA. PhrdB was used as negative control. (C) ChIP-qPCR assays in vivo. Anti-ScbR2 antibodies were used to immunoprecipitate ScbR2–DNA complexes from 30-, 51-, and 60-h cultures treated with formaldehyde. IgG-immunoprecipitated complexes were used as the negative control. The y axis represents the relative enrichment of PredD and PadpA compared with the control. The relative values are means ± SD from three independent experiments.