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Sas4−/− mouse embryos arrest at mid-gestation. (A) Dorsal view of wild-type and Sas4−/− embryos at E8.5. (B) Wild-type and Sas4−/− embryos at E9.5. (C and D) Cross-sections of wild-type and Sox2-Cre; Sas4fl/- embryos at E8.5 stained for E-cadherin (red). Arrows indicate the neural plate (ectoderm), arrowheads indicate the mesenchyme (mesoderm), and asterisks indicate the gut (endoderm). (Scale bars: 300 µm.)
