Table 1.

Limiting dilution analysis of precursor potential of TCR-transduced DN3 cells

	αβ-Lineage*		γδ-Lineage*
TCR/stromal cell	1/freq	(95% C.L.)	1/freq.	(95% C.L.)
TCR-β/OP9-DL4	1.05	(0.73–1.52)	Nil	N/A
TCR-β/B/c-DL4	1.02	(0.72–1.46)	Nil	N/A
TCR-γδ/OP9-DL4	116.23	(49.97–270.39)	1.35	(0.95–1.91)
TCR-γδ/B/c-DL4	47.56	(26.81–84.35)	3.38	(2.40–4.77)
TCR(β+γδ)/OP9-DL4	353.00	(84.36–1,477.09)	1.39	(0.98–1.97)
TCR(β+γδ)/B/c-DL4	233.99	(72.38–756.47)	1.67	(1.18–2.36)

^*αβ-Lineage outcome was determined by the detection of CD4 and CD8 expressing cells, with >50 cells per well analyzed. γδ-Lineage outcome was determined by the presence of CD4 and CD8 DN cells that expressed high levels of CD73, with >30 cells per well analyzed. C.L., confidence limits for P ≤ 0.05; N/A, not applicable. Limiting dilution analyses coculture conditions and TCR used for transductions of Rag2^−/− DN3 cells are indicated in SI Methods. Numbers in bold represent frequencies of respective lineage outcomes for KN6-transduced DN3s exposed to weak ligand in the absence and presence of TCR-β, respectively.