Fig. 2.

Read-through of a premature stop codon is undetectable by radio-immunoprecipitation and Western blot. (A) LKb cells infected with the indicated VV were ³⁵S-labeled and incubated ± 300 μg/mL of gentamicin. Autoradiography of immunoprecipitated NP demonstrates 36-kDa truncated NP, resulting from termination at the PTC, in the negative control (NEG) and 56-kDa full-length NP in the positive control (POS). No full-length protein was detectable from any of the test constructs using this approach with a range of gentamicin concentrations. (B) LKb cells were cultured ± 900 μg/mL of gentamicin and infected with the indicated VV before assessing the presence of 56-kDa NP by anti-NP Western blot. Expression of full-length NP was not observed from any stop codon construct.