5. PMN are the primary source of BPI in neonatal TAs.
Representative flow cytometry of neonatal TA cells (28 week gestational age male): (A) stained with both FITC- and PE-labeled isotypes, with background staining set at 1%. (B) staining with FITC-labeled granulocyte cell surface antigen CD66b and intracellular PE-labeled BPI. Most CD66b+ cells were also BPI+. Percentages of the total amount of cells are given within the charts for each quadrant. (C) The histograms depict a shift in median fluorescence for CD66b positive cells (shaded) versus isotype (dashed line) and (D) BPI positive cells (shaded) versus the isotype (dashed line).