Figure 2.

Polymerase chain reaction-restriction fragment length polymorphism analysis of selected strains of Leishmania pecies using Hae III restriction enzyme. Lanes: M–100 bp ladder (Fermentas); (1) Leishmania major (MHOM/IR/75/ER); (2) Leishmania tropica (MHOM/IR/03/Mash-878); (3) Leishmania donovani (IPER/IR/2007/HS10); (4) Leishmania infantum (positive control); (5) Obtained isolate from patient. Standard species were taken from reference laboratory of the Parasitology Department, Tehran University of Medical Sciences