Skip to main content
. 2013 Oct 30;44(1):127–136. doi: 10.1002/eji.201343593

Figure 1.

Figure 1

Patients who develop TB-IRIS express increased levels of MMPs. Log-fold induction of MMP genes by heat killed MTB in PBMCs from TB-IRIS at the time of TB-IRIS and non-TB-IRIS control participants who had received a similar duration of antitubercular and antiretroviral therapy. PBMCs from 22 TB-IRIS and 22 controls were cultured in the presence or absence of heat killed H37Rv M. tuberculosis for 6 and 24 h. The PBMCs were lysed and mRNA analysis was performed by quantitative RT-PCR. Transcript abundance was calculated by subtracting the Ct β-Actin from the CT of the MMP gene of interest. Fold induction was calculated by the ΔΔCt method and values normalized by Log-10 transformation. Fold induction analysis between IRIS and non-IRIS was performed by unpaired t-test. At 6 h, after Bonferroni correction, MMP gene induction was significantly higher in TB-IRIS than in controls for MMP-3, MMP-7, and MMP-10 (pcorr ≤ 0.05). At 24 h, significant upregulation existed between TB-IRIS and non-IRIS participants for MMP-1, MMP-7, MMP-10, and MMP-12. MMP-12 was undetectable in 6 h cultures. In this figure, the black bars represent TB-IRIS patients while open bars represent non-IRIS controls. Data are shown as median + IQR of 22 TB-IRIS versus 22 non-IRIS control patients for each MMP analyzed (as represented by each panel).