(A) Images from time-lapse series showing cellular contraction after MOMP displaying two distinct phenotypes. An example of rapid contraction and cellular breakup is shown in the upper series of images. An example of slower contraction after MOMP.
(B) Quantification of the percent of cells displaying slow contraction after MOMP defined as cell area ≥60% of the original area 20 minutes after MOMP/incomplete MOMP. Cells were treated with trehalose in the presence or absence of ATG5 or ATG7 shRNAs as indicated. Trehalose increases the number of slow contracting cells and this is abolished by autophagy inhibition.
(C) Continued monitoring of fast contracting cells after MOMP shows irreversible cell breakup and death from which they cannot recover.
(D) Continued monitoring of slow contracting cells shows that they can recover normal morphology hours after MOMP.