Fig. (2).

Cell viability assay to test the Anti-cancer Activity of Egyptian Artemisia, EA and Jericho Artemisia, JA extracts.

Human Breast Adenocarcinoma MCF7 (BA), Human Lung Carcinoma (LC) and Chinese Hamster Ovary (CHO) cell lines and Primary Human Dermal Fibroblasts isolated from adult skin (HDFa) cells were used. 104 cells /ml of each cell type was incubated separately with A. EA and JA leaves’ water extracts (500mg/ml), B. EA and JA leaves’ methanol extracts (500mg/ ml), C. EA and JA leaves’ hexane-chloroform extracts (500mg/ml), and 70% DMSO in the growth media as positive control. Alamar Blue® reagent was used to determine the cell viability percentages. Readings were taken using the micro plate spectrophotometer (Absorbance at 570 nm and 600 nm wavelengths).