Fig. 2.

Agarose gel electrophoresis of PCR products of a 265-bp fragment of pcDNA3.1/V5-His plasmid using the Pfx DNA polymerase. PCR was performed using primers from Table 1. Lane 1 T7 forward primer+reverse primer; lane 2 forward primer with one mismatch on the 3′ end+reverse primer; lane 3 forward primer with two mismatches on the 3′ end+reverse primer; lane 4 forward primer with three mismatches on the 3′ end+reverse primer