s,n,w |
Subscripts: s, solute; n, non-permeating; w, water |
None |
None |
Lp
|
Hydraulic conductivity in the presence of cryoprotectant |
μm min-1 atm-1
|
Parameter |
Ps
|
Solute permeability |
μm min-1
|
Parameter |
Ea
|
Activation energy |
kcal mol-1
|
Parameter |
T |
Temperature |
K |
295 |
A |
Cell surface area |
μm2
|
Parameter |
V |
Total cell volume |
μm3
|
Variable |
Vb
|
Osmotically inactive cell volume |
μm3
|
Parameter |
W |
Intracellular water volume |
μm3
|
Variable |
S |
Moles of internal permeating solute at time t
|
Moles |
Variable |
t |
Time |
Seconds |
Variable |
πiso
|
Osmolality of initial (isotonic) intracellular nonpermeating solutes |
Osm kg-1 H2O |
0.290 |
πn
|
Osmolality of extracellular nonpermeating solutes |
Osm(kg H2O)-1
|
Variable |
Ms
|
Molality of extracellular permeating solutes |
Mol (kg H2O)-1
|
Variable |
N |
Osmoles of intracellular salts |
Osm |
Variable |
V̄ |
Partial molal volume of solute |
L mole-1
|
Variable |
R |
The universal gas constant |
kcal mole-1 K-1
|
1.987 × 10-3
|