Table 3.
Room temperature hydraulic conductivity (Lp) for five mouse embryonic stem cell (mESC) lines in the presence of 1.0 M cryoprotective agent (CPA).
| Genetic background | Dimethyl sulfoxide | Ethylene glycol | 1,2-propanediol | Glycerol |
|---|---|---|---|---|
| C57BL/6* | 0.41 ± 0.03 | 0.48 ± 0.06 | 0.42 ± 0.03 | 0.14 ± 0.01 |
| BALB/c | 0.15 ± 0.01a,1 | 0.15 ± 0.01a,1 | 0.18 ± 0.01a,1 | 0.05 ± 0.01 b,1 |
| CBA | 0.21 ± 0.01a,1 | 0.15 ± 0.01a,b,1 | 0.19 ± 0.01a,1 | 0.09 ± 0.01b,2 |
| FVB | 0.39 ± 0.04a,2 | 0.24 ± 0.03b,1,2 | 0.22 ± 0.04b,1,2 | 0.10 ± 0.01c,2 |
| 129R1 | 0.53 ± 0.12a,2 | 0.35 ± 0.02a,2 | 0.33 ± 0.04a,2 | 0.10 ± 0.01b,2 |
Hydraulic conductivity values are expressed as mean ± SEM μm·min-1·atm-1. Different superscripts indicate statistically significant effects (p<0.05): of CPA within each genetic background on Lp values (letters, rows), or of genetic background within each CPA group on Lp values (numbers, columns).
Values for the C57BL/6 mESC line are previously published [35] and listed for ease of comparison, but were not included in statistical analyses.