Table 4.
Room temperature cryoprotectant permeability (Ps) for five mouse embryonic stem cell (mESC) lines in the presence of 1.0 M cryoprotective agent (CPA).
| Genetic background | Dimethyl sulfoxide | Ethylene glycol | 1,2-propanediol | Glycerol |
|---|---|---|---|---|
| C57BL/6* | 4.59 ± 0.41 | 4.17 ± 0.24 | 6.58 ± 0.38 | 1.05 ± 0.07 |
| BALB/c | 8.62 ± 0.53a,1 | 11.74 ± 0.84b,1 | 10.51 ± 0.67a,b,1 | 1.99 ± 0.251,c |
| CBA | 9.10 ± 0.60a,1 | 8.41 ± 0.28a,1 | 9.40 ± 0.61a,2 | 2.32 ± 0.281,b |
| FVB | 4.53 ± 0.37a,2 | 5.32 ± 0.50a,2 | 6.92 ± 0.64a,3 | 1.33 ± 0.371,b |
| 129R1 | 4.00 ± 0.48a,2 | 3.92 ± 0.23a,2 | 5.24 ± 0.27a,4 | 0.99 ± 0.071,b |
Cryoprotectant permeability values are expressed as mean ± SEM μm·min-1. Different superscripts indicate statistically significant effects (p<0.05): of CPA within each genetic background on Ps values (letters, rows), or of genetic background within each CPA group on Ps values (numbers, columns).
*
Values for the C57BL/6 mESC line are previously published [35] listed for ease of comparison but were not included in statistical analyses.