Table 5.

Activation energies of hydraulic conductivity ( $E_{a}^{L_{p}}$ ) and of cryoprotectant permeability ( $E_{a}^{P_{s}}$ ) for five mouse embryonic stem cell (mESC) lines in the presence of 1.0 M cryoprotective agent (CPA).

Genetic background

E_{a}^{L_{p}}

E_{a}^{P_{s}}

Me₂SO

C57BL/6^*

14.29

15.35

14.12

15.47

13.19

14.08

BALB/c

12.33 ± 0.60^a,1,2

12.94 ± 0.49^a,1

13.80 ± 0.52^a,1

13.44 ± 0.81^a,1

13.04 ± 0.21^a,1

15.15 ± 1.11^a,1

CBA

10.82 ± 1.61^a,1

13.01 ± 1.09^a,1

11.51 ± 0.45^a,1

17.35 ± 2.37^a,1

16.75 ± 1.16^a,1

19.79 ± 0.46^a,1

FVB

12.29 ± 1.42^a,1,2

13.01 ± 1.09^a,1

10.09 ± 1.34^a,1

16.94 ± 1.47^a,1

14.95 ± 0.66^a,1

19.00 ± 1.99^a,1

129R1

16.82 ± 1.98^a,2

14.52 ± 0.50^a,1

12.94 ± 1.03^a,1

13.80 ± 1.78^a,1

15.85 ± 0.63^a,1

18.17 ± 0.70^a,1

Activation energy is expressed as mean±SEM kcal·mol^-1. Different superscripts indicate statistically significant effects (p<0.05) (i.e., similar superscripts indicate statistically indistinguishable groups, p<0.05): of CPA within each genetic background on $E_{a}^{L_{p}}$ or $E_{a}^{P_{s}}$ values (letters, rows), or of genetic background within each CPA group on $E_{a}^{L_{p}}$ or $E_{a}^{P_{s}}$ values (numbers, columns).

Values for the C57BL/6 mESCline are previously published values [35] listed for ease of comparison but were not included in statistical analyses. Me₂SO, dimethyl sulfoxide; EG, ethylene glycol; PG, 1,2-propanediol.