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. Author manuscript; available in PMC: 2015 Apr 17.
Published in final edited form as: FEBS Lett. 2014 Jan 14;588(8):1297–1303. doi: 10.1016/j.febslet.2013.12.024

Figure 2.

Figure 2

Photomicrographs show the distribution of immunoreactive Cx50 in HLE-Cx50 (A) and HLE-Cx50P88S (B). Arrows indicate staining at appositional membranes consistent with the expected distribution of gap junctions; this staining is prominent in cells expressing Cx50, but not in those expressing Cx50P88S. Arrowheads indicate cytoplasmic accumulations seen only in cells expressing Cx50P88S. C-E. Immunoblots show levels of unmodified and glycosylated CADM1 (C), NQO1 (D) and MURF2 (E) in total homogenates of untransfected HLE cells or cells transfected with Cx50 or Cx50P88S. Levels of CADM1 were decreased in HLE-Cx50P88S compared to untransfected and HLE-Cx50 cells and levels of NQO1 were increased in HLE-Cx50 compared to untransfected and HLE-Cx50P88S cells. Levels of MURF2 were similar between HLE-Cx50 and HLE-Cx50P88S.