Plasma prolactin levels of female ACI rats treated with or without a silastic E2 implant along with either two sham polymeric implants (2 cm), or two curcumin implants (2 cm, 20% load) or curcumin diet (1,000 ppm) for 3 weeks (n=6) (A), 3 months (n=6) (B) and 6 months (n=6–8) (C). Plasma prolactin was measured by using an EIA kit following manufacturer’s protocol and a p value of <0.05 was considered significant. Groups compared are denominated with the same alphabets (* p<0.05, ** p<0.01 and *** p<0.001).