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. Author manuscript; available in PMC: 2015 Mar 1.
Published in final edited form as: Alcohol. 2014 Jan 7;48(2):99–111. doi: 10.1016/j.alcohol.2013.12.001

Fig. 1.

Fig. 1

Alcohol-induced apoptosis indicated by immunocytochemistry of cleaved-caspase-3, shown in photomicrographs of saline-treated (left panels) and alcohol-treated (right panels) mice for cingulate cortex (top panels), striatum (middle panels), and hippocampal formation (bottom panels). Alcohol was administered to B6 mice on PD 7 (2.5 g/kg twice a day, 2 h apart, s.c.) and the pups were perfused for immunocytochemistry 6 h after the second alcohol injection. In saline-treated mice, very few cleaved-caspase-3 positive neurons were seen (A, C, E; arrows). In contrast, many caspase-3-positive neurons were evident in the cingulate cortex (B) [as well as parietal, temporal and retrosplenial cortices (not shown)], striatum (D), and hippocampal formation CA1/2 [as well as subiculum (not shown)] (F) of mice treated with alcohol. Scale bars: A, B, E, F = 100 μm; C, D = 30 μm.