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Obstacle shape and position control the evolution of microtissue morphology. Three-ring microtissues were formed in a series of different molds. Each mold had three obstacles, each in the form of either a post (P) or a cone (C). The left to right order of the obstacles is labeled in the left margin. Molds were seeded with NHFs, and time lapse phase-contrast images taken at time zero when the cells had settled (A, C, E) and 24 h later (B, D, F) are shown. Scale bar is 500 μm.
