FIG. 8.

Obstacle position and shape control release of honeycomb microtissues. Molds designed to form a large honeycomb tissue were seeded with NHFs, and phase-contrast images were taken to document the obstacle-mediated morphological changes that result in the release of the honeycomb from the mold. One honeycomb design had three cones in a row along one outer edge of the honeycomb. Images at 34 h (A), 40 h (B), and 46 h (C) after seeding show that honeycomb release starts at the edge containing the three cones. Another honeycomb design had a single cone in the center and 12 cones around the outer edge. Images at time zero (D), 4 h (E), and 24 h (F) after seeding show that honeycomb release occurs in a circumferential pattern. The diamonds and stars in (C) and (D) indicate posts and cones, respectively. Scale bar is 1000 μm. Color images available online at www.liebertpub.com/tea