Table 1.

Elements of Mold Design That Influence Morphological Evolution

Schematic of design elements	Design feature	How design element controls morphological evolution
	Rod length	Shorter rods more stable, predictable time to rupture, predictable point of rupture, rupture point dependent on width, no rupture for some cell types
	Rod width	Wider rods more stable, predictable time to rupture, influences point of rupture, no rupture for some cell types (e.g., H35)
	Post diameter	Creates circumferential tension around post, creates uniaxial tension between two posts, controls diameter of microtissue
	Post height	Minimum height needed for stability
	Cone diameter	Dictates starting point of circumferential tension
	Cone height	Dictates time until tissue is released from mold
	Cone slope	Dictates rate at which tension is dissipated
	Placement of obstacles	Controls overall size and shape of microtissue, dictates lines of tension in microtissue, controls when and where tension is released