FIG. 3.

Cytotoxicity of PYE and its solvent partitioned fractions in vitro. Peritoneal macrophages were isolated from thioglycollate-injected mice and cultured in the presence of PYE, water, ethyl acetate, or chloroform for 24 h. Extracellular lactate dehydrogenase (LDH) release was presented as optical density. Each bar represents means±SD of four independent experiments. (A) PYE, (B) water fraction, (C) chloroform fraction, and (D) ethyl acetate fraction. *P<.05, **P<.01 compared with untreated cells.