FIG. 4.

Effect of PYE and its fractions on LPS-induced TNF-α and IL-6 gene expression. Peritoneal macrophages were pretreated 1 h with PYE (A) or its fractions (B) and (C), and then stimulated for 4 h with LPS (100 ng/mL); 1 μM dexamethasone was used as a reference drug. Total RNA was extracted and real-time polymerase chain reaction (PCR) was performed. GAPDH was used as an internal control. Data are expressed as means±SD of three independent experiments. *P<.05, **P<.005, ***P<.001 compared with cells treated with LPS only (control).