Effect of water, chloroform, and ethyl acetate fractions from PYE on LPS-induced IκBα degradation and MAPK activation. Peritoneal macrophages were pretreated 1 h with 1 μM dexamethasone (Dex), 200 μg/mL water fraction (W), 10 μg/mL chloroform fraction (Ch), and 200 μg/mL ethyl acetate fraction (EA), and then stimulated for 4 h with LPS. Whole protein was extracted and examined by Western blot analysis. Tubulin was used as an internal control. One of the three experiments is shown.