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. 2014 Mar 28;192(9):4233–4241. doi: 10.4049/jimmunol.1302850

FIGURE 3.

FIGURE 3.

TLR2 agonist–conjugated peptides were internalized by Rab7+ late endosomes and generated the epitope in endolysosomes. BMDCs were incubated with 1 μM FITC-conjugated IDG or Pam2IDG (green) for 10, 20, or 30 min at 37°C. To detect endosomes, BMDCs were stained with anti-EEA1 (A, C) or anti-Rab7 (B, D) to visualize EEA1+ and Rab7+ endosomes (red), respectively. (E and F) The colocalization rates of IDG-FITC and Pam2IDG-FITC colocalized with EEA1+ and Rab7+ endosomes. WT (G) or TAPKO (H) BMDCs were incubated with 1 μM Pam2EQL for 30 or 60 min. After incubation, the cells were double stained with 25.D1-16 Ab (red) to detect H2-Kb/OVA257–264 and with anti-Rab7 or anti-LAMP1 (green). Colocalization is depicted in yellow. The data shown are representative of two independent experiments. **p < 0.01.