FIGURE 5.
Rab7 overexpression could promote Pam2EQL cross-presentation by BMDCs. WT (A) or TAPKO (B) BMDCs were transduced with Lenti-vector, Lenti-Rab7, or Lenti-Rab7T22N for 2 d before being peptide pulsed. The transduced BMDCs were pulsed with 1 μM Pam2IDG or Pam2EQL for 2.5 h at 37°C. After free peptides were washed away, 2 × 104 BMDCs were cocultured for 48 h with 2 × 105 CD8+ T cells that were purified from the splenocytes of OT-I–transgenic mice. Cell proliferation was determined using a [3H]thymidine incorporation assay. The data shown are representative of two independent experiments. **p < 0.01.