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. 2014 May 1;20(13):1964–1976. doi: 10.1089/ars.2013.5286

FIG. 7.

FIG. 7.

β3-endonexin diminishes endothelial proliferative responses under hypoxia. HMEC-1 cells were transfected with expression vectors coding for either β3-endonexin short (EN-S) or long (EN-L) isoform or with control vector (Ctr), or with siRNA against β3-endonexin (siEN) or scrambled RNA (siCtr). Alternatively, HMEC-1 cells were transfected with siRNA against β3-endonexin (siEN) or scrambled RNA (siCtr) and, in addition, transfected with an expression vector coding for IκBdn or with control vector (Ctr) or with siRNA against HIF-1α (siHIF-1α). Cells were exposed to hypoxia (1% oxygen, Hx) for 24 h or remained under normoxia (Ctr), and BrdU incorporation assay was performed. Normoxic controls were set equal to 100% (n=3, *p<0.05 vs. normoxic Ctr/siCtr; #p<0.05 vs. hypoxic Ctr/siCtr). Western blot analyses were performed with antibodies against IκBα and HIF-1α. β-actin levels served as loading control. BrdU, 5-bromo-2′deoxyuridine.