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HCASMC-infiltrated 3D polycarbonate urethane (PCU) scaffolds in long-term cultures. HCASMCs were seeded on 3D porous scaffolds and were cultured for 8 and 16 days. Images by confocal microscopy taken at different focal planes after staining for F-actin (green) showed that cells were both migrating and proliferating as the culture time increased. Nuclei are labeled blue. Scale bar=100 μm. Color images available online at www.liebertpub.com/tea
