FIG. 2.

Western blot for confirmation of samples that tested positive in competitive enzyme-linked immunosorbent assays (cELISA) but not in the microagglutination (MA) tests (n=7). Each well contains F. tularensis lipopolysaccharide (LPS) as an antigen. Typical LPS ladder-like bands were present in six of the seven samples positive in cELISA but not in the MA test. The serum sample from a mouse (lane 6) was negative in western blot. Lanes 1–5, 6, and 7 show the western blot patterns of the blood samples obtained from Japanese black bears, a small rodent and a Japanese raccoon dog. Lanes 8 and 9 show the western blot pattern of hyperimmune rabbit (positive control), and a normal rabbit (negative control) serum samples, respectively.