FIG. 1.

A schematic diagram of the protocol for mesodermal lineage induction of EBs derived from hESCs and subsequent differentiation to the osteogenic and chondrogenic lineages. To induce mesodermal induction of EBs, EBs composed of hESCs were cultured in the hMSC-CM. EB culture with EB media served as a control. Subsequently, the EBs were induced to undergo osteogenic or chondrogenic differentiation. CM, conditioned medium; EB, embryoid body; hESC, human embryonic stem cell; hMSC, human mesenchymal stem cell. Color images available online at www.liebertpub.com/tea