Enhanced mesodermal lineage induction of hESC EBs through the treatment of EBs with the hMSC-CM for 4 days. The gene expressions of mesodermal (T, WNT3, and MIXL1), endodermal (FOXQ1, GSC, and CER), and ectodermal (ZIC1 and SOX1) of hMSC-CM-treated EBs (hMSC-CM EBs) were determined by qRT-PCR and normalized to the levels of the untreated EB group (n=4, *p<0.05 vs. untreated EB). qRT-PCR, quantitative real-time reverse transcription–polymerase chain reaction. Color images available online at www.liebertpub.com/tea