Table 1.

Human-Specific Primers for Each Gene

Gene	Primer
GAPDH	Sense 5′-GTC GGA GTC AAC GGA TTT GG-3′
	Antisense 5′-GGG TGG AAT CAA TTG GAA CAT-3′
ZIC1	Sense 5′-GCG CTC CGA GAA TTT AAA GA-3′
	Antisense 5′-CGT GGA CCT TCA TGT GTT TG-3′
SOX1	Sense 5′-TAC AGC CCC ATC TCC AAC TC-3′
	Antisense 5′-GCT CCGACT TCA CCA GAG AG-3′
FOXQ1	Sense 5′-GCG CGG ACT TGC ACT TT-3′
	Antisense 5′-GCA CGT TTG ATG GAG ATT TTA AAA-3′
GSC	Sense 5′-GAG GAG AAA GTG GAG GTC TGG TT-3′
	Antisense 5′-CTC TGA TGA GGA CCG CTT CTG-3′
CER	Sense 5′-ACA GTG CCC TTC AGC CAG ACT-3′
	Antisense 5′-ACA ACT ACT TTT TCA CAG CCT TCG T-3′
T	Sense 5′-ATC ACA AAG AGA TGA TGG AGG AA-3′
	Antisense 5′-GGT GAG TTG TCA GAA TAG GTT GG-3′
WNT3	Sense 5′-CTG CCA GGA GTG TAT TCG CAT C-3′
	Antisense 5′-GAG AGC CTC CCC GTC CAC AG-3′
MIXL1	Sense 5′-GGT ACC CCG ACA TCC ACT T-3′
	Antisense 5′-TGG AAG GAT TTC CCA CTC TG-3′
Alkaline phosphatase	Sense 5′-CCC TTG ACC CCC ACA ATG T-3′
	Antisense 5′-GTT GTT CCT GTT CAG CTC GTA-3′
Collagen type I	Sense 5′-CAG CCG CTT CAC CTA CAG C-3′
	Antisense 5′-TTT TGT ATT CAA TCA CTG TCT T-3′
Osteopontin	Sense 5′-GCC GAC CAA GGA AAA CTC ACT-3′
	Antisense 5′-CAG AAC TTC CAG AAT CAG CCT-3′
Osteonectin	Sense 5′-TCC ACA GTA CCG GAT TCT CTC T-3′
	Antisense 5′-TCT ATG TTA GCA CCT TGT CTC CAG-3′
Osteocalcin	Sense 5′-CCT CAC ACT CCT CGC CCT ATT-3′
	Antisense 5′-CCC TCC TGC TTG GAC ACA AA-3′
Collagen type II	Sense 5′-ATA AGG ATG TGT GGA AGC CG-3′
	Antisense 5′-TTT CTG TCC CTT TGG TCC TG-3′
Aggrecan	Sense 5′-ACC CTG GAA GTC GTG GTG AAA-3′
	Antisense 5′-CGT GGC AAT GAT GGC ACT G-3′