FIG. 7.

hADSCs were induced into (A) NLCs with morphology changing continuously as time lapse from day 0 (D0) to day 6 (D6). (B) Without any treatment, hADSCs positively express Tuj1 and MAP2 and negatively express NeuN, Synapsin1/2, and vGAT. After 3-day incubation with cocktail medium, most hADSCs-NLCs positively expressed Tuj1, MAP2 but not Synapsin1/2 (C). After additional 3-day incubation with the cocktail medium supplemented with neurotrophic factors, most cells began to express neuron function-related markers of NeuN, Synapsin, and vGAT (D). qRT-PCR data demonstrated that neuron markers such as NFL, NFM, as well as function- and ion channel-related genes such as Kv4.2, NE-Na, and CACNA1G were all upregulated (E, F). Scale bar=25 μm for (A), scale bar=50 μm for (B–D). Color images available online at www.liebertpub.com/tea