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. 2014 Mar 21;20(7-8):1271–1284. doi: 10.1089/ten.tea.2012.0773

FIG. 8.

FIG. 8.

hADSCs cultured on the 3D scaffold were subjected to the neuron cocktail medium with similar operation on the plastic dishes. After 6 day differentiation, hADSC-NLCs were detected the morphology by SEM (A). To monitor hADSC proliferation capability on this 3D scaffold material, MTT assay were carried out within 96 h at a time course of 24 h (B). Both hADSC-NLCs and hADSCs on 3D scaffold at D6 were subjected to trizol digestion for total RNA extraction. qRT-PCR were carried out to detect the neuron-related gene expression variance (C). ADSC-Ma means hADSCs cultured on the 3D scaffold material to compare with the hADSCs cultured on the plastic dishes. Two-way ANOVA with Bonferroni post-tests were performed for the data in (B) using GraphPad Prism version 5.00 for Windows, GraphPad Software (San Diego, CA), www.graphpad.com. *p<0.05.